10 (1) 05
Nauka innov. 2014, 10(1):43-55
https://doi.org/10.15407/scin10.01.043
O.V. Markovskyi1, M.O. Bannikova1, V.V. Borisova2, T.V. Fedorenko1, B.V. Morgun1
1 Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Kyiv
2 Institute of Steppe Zone Agriculture, NAAS of Ukraine. Dnipropetrovsk
Detection of Genes that Determine Maize Grain Quality Characteristics and Resistance to Stress Factors
Section: Scientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
Abstract: 200 experimental maize samples (Maize Company) were examined for the presence of genes that determine the quality characteristics of grain (wx and fl-2 genes), herbicide (bar (pat), epsps genes) and insect (cry-genes) resistance. The total DNA was extracted from maize living plant tissue. Primers to detect wx, fl-2, bar (pat), mepsps, CP4 epsps, cry1A(b), cry1F, cry1A.105, mcry3A, cry2Ab2, cry3Bb1, cry34Ab1, cry35Ab1 genes were designed and selected. Multiplex and Touchdown PCR were worked out. PCR amplification of certain sequences was carried out. No transgenes (bar (pat), mepsps, CP4 epsps, cry1A(b), cry1F, cry1A.105, mcry3A, cry2Ab2, cry3Bb1, cry34Ab1, cry35Ab1) were found among 200 analyzed experimental maize samples. At the same time, fl-2 gene was found in 41 samples, wx gene was found in 192 analyzed samples.
Key words: maize, transgenes, grain quality characteristics, primers, polymerase chain reaction (PCR).
Full Text (PDF)
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