Nauka innov. 2005, 1(3):70-75
https://doi.org/10.15407/scin1.03.070

S. S. Maliuta, G. D. Telegeev, M. V. Dybkov, D. O. Miroshnychenko, G. V. Yelska
Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv

 

Development of Test-System for Ph-Leukaemia Diagnostics by Polymerase Chain Reaction

Section: Scientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
Abstract: The hallmark of tumor growth for the majority of patient with chronic myelogenous leukemia is Philadelphia chromosome (Ph') – a product of reciprocal translocation of 9 and 22 chromosomes. At the molecular level it is connected with bcr/abl-fusion gene. This paper presents data of development of national test-system for detection of hybrid gene with PCR method. It was shown that specific combination of primers used allow to advance a probability to obtain the correct data.
Key words: Ph-positive leukaemia, BCR/ABL, diagnostics, PCR.

References:
1. Butenko Z.A., Gluzman D.F., Zak K.P. i dr. Citohimija i jelektronnaja mikroskopija kletok krovi i krovetvornyh organov. Kiev: Naukova dumka, 1974 [in Russian].
2. Gluzman D.F., Bebeshko V.G., Nadgornaja V.A. i dr. Jembrional'noe krovetvorenie i gemoblastozy u detej. Kiev: Naukova Dumka, 1988 [in Russian].
3. Pinchuk V.G., Gluzman D.F., Nadgornaja V.A. i dr. Immunocitohimicheskie metody i monoklonal'nye antitela v onkogematologii. Kiev: Naukova dumka, 1990 [in Russian].
4. Boehm, T.H. Rabbits A chromosomal basis of lymphoid malignancy in man. E.J.Biochem, 1989, 185:1–17 [in English].
5. M.J. Cline The molecular basis of leukemia. The New England Journal of Medicine, 1994, 330(5):328–336 [in English].
https://doi.org/10.1056/NEJM199402033300507
6. Rabbits T.H. Chromosomal translocations in human cancer. Nature, 1994, 372(6502):143–149 [in English].
https://doi.org/10.1038/372143a0
7. Nowell PC, Hunderford DA. A minute chromosome in human chronic granulocytic leukemia. Science, 1960, 132:1497–1499 [in English].
8. Rowley JD. A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature, 1973, 243(5405):290–293 [in English].
https://doi.org/10.1038/243290a0
9. Telegeev G.D., Dybkov M.V., Karpenko O.I., Cherepenko E.I. Molekuljarnye osnovy Ph-lejkemij i puti ih lechenija. Biopolym. Cell., 1994, 10(5):78–92 [in Russian].
https://doi.org/10.7124/bc.0003BD
10. Gishizky ML. Molecular mechanisms of Bcr-Ablinduced oncogenesis. Cytokines Molular Therapy, 1996, 2(4):251–261 [in English].
11. Southern E.M. Detection of specific sequences among DNA fragments separated by gel electroforesis. J. Mol.Biol., 1975, 98(3):503–517 [in English].
https://doi.org/10.1016/S0022-2836(75)80083-0
12. G.Grosveld, T.Verwoerd, T van Agthoven et al. The chronic myelocytic cell line K 562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Molecular and Cellular Biology, 1986, 6(2):607–616 [in English].
13. E.S.Kawasaki, S.S.Clark, M.Y.Coyne et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by deletion of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA, 1988, 85(15):5698–5702 [in English].
https://doi.org/10.1073/pnas.85.15.5698
14. Maurer J., Janssen J., Thiel E., et al. Detection of chimeric BCR ABL genes in acute lymphoblastic leukemia by the polymerase chain reaction. The Lancet., 1991, 337(8749):1055–1058 [in English].
https://doi.org/10.1016/0140-6736(91)91706-Z
15. Tkachuk D.C., Westbrook C.A., Andreeff M., et al. Detection of bcr-abl fusion in chronic myelogenous leukemia by in situ hybridization. Science, 1990, 250(4980):559–562 [in English].
https://doi.org/10.1126/science.2237408
16. N.Testori, G.Martinelli, P.Farabedoli et al. A new method of "in-cell reverse transciptase-polymerase chain reaction" for detection of BCR/Abl transcripts in chronic myeloid leukemia patients. Blood, 1996, 87(9):3884–3892 [in English].
17. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinum thiocyanate-phenol-chloroform extraction. Analyt. Biochem, 1987, 162:156–159 [in English].
https://doi.org/10.1016/0003-2697(87)90021-2