8(5)08

Nauka innov. 2012, 8(5):72–78
https://doi.org/10.15407/scin8.05.078

V.Yu. Yatsyshyn1, M. Kluz2, D.V. Fedorovych1, А.А. Sibirny1,2
1Institute of Cell Biology, NAS of Ukraine, Lviv
2Rzeszowski Uniwersytet, Poland

 

Construction of the Overproducers and Development of Technology for Flavin Mononucleotide Production Based on Yeast Candida famata

Section: Scientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
Abstract: The strains of the yeast Candida famata containing 5-6 copies of FMN1 gene (encoding riboflavin kinase) were constructed. The optimal conditions for flavin mononucleotide (FMN) synthesis under batсh and feed-batсh cultivation were investigated. The medium components and cultivation conditions for maximal accumulation of product – FMN were optimized.
Key words: flavin mononucleotide (FMN), yeast, Candida famata, oversynthesis, overproducers.