Construction of the Recombinant Yeast Strain of Pichia stipitis with Improved Alcoholic Fermentation of Xylose

1Dmytruk, KV, 1Kurylenko, OO, 1Ishchuk, OP, 1Ubiyvovk, VM, 2Sibirny, VA, 1Fedorovych, DV, 3Sibirny, AA
1Institute of Cell Biology, NAS of Ukraine, Lviv
2Rzeszowski Uniwersytet, Rzeszów, Poland
3Institute of Cell Biology, NAS of Ukraine, Lviv; Rzeszowski Uniwersytet, Rzeszów, Poland
Nauka innov. 2010, 6(6):45-50
Section: Scientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
The modified form of the xylose reductase (XR) of Pichia stipitis with decreased affinity toward NADPН(H+) was created via site-specific mutagenesis. Recombinant strain of P. stipitis overexpressing the mutated XR characterized by 1,3-fold increase in productivity of xylose alcoholic fermentation was constructed. The rates of alcoholic fermentation of hydrolyzates of wheat bran and sawdust were also improved.
Keywords: alcoholic fermentation, ethanol, Pichia stipitis, xylose, yeast
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