Development of Immunochemical Test Systems for Anti-Diphtheria Immunity Control in Population of People

1Kaberniuk, АА, 1Oliinyk, OS, 1Redchuk, ТА, 1Romanyuk, SІ, 1Kolibo, DV, 2Ievtushenko, VV, 3Golovach, OV, 2Kramarev, SO, 1Komisarenko, SV
1O.V. Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv
2Department of Pediatric Infectious Diseases of the O.O. Bohomoltsia National Medical University. AA Bogomoletz, Kyiv
3Kyiv City Children's Clinical Infectious Diseases Hospital, Kyiv
Nauka innov. 2008, 4(3):22-31
Section: Scientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
Diagnostic test-systems for rapid and effective assessment of antidiphtheria immunity are developed. The prototypes of immunochemical test-systems based on enzymelinked immunosorbent and immunochromatographic assays for detection of antibodies to diphtheria toxin subunits A and B in human serum are presented. It was suggested to use recombinant subunits of diphtheria toxin as antigens that allowed to define the specificity of anti-diphtheria antibodies in human sera.
Keywords: diphtheria, diphtheria toxin's recombinant subunits, elisa, immunochromatography, protective immunity evaluation
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