9(5)09
Nauka innov. 2013, 9(5):69-77
https://doi.org/10.15407/scin9.05.069
S.P. Ponomarenko1, V.A. Tsygankova2, Ya.B. Blume3, A.P. Galkin3
1 Interdepartmental Scientific and Technological Center "Agrobiotech", NAS and MES of Ukraine, Kyiv
2 Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Kyiv
3 Institute of Food Biotechnology and Genomics Sciences, NAS of Ukraine, Kyiv
New Trend in Crop Production – Application of Plant Natural Multicomponent Growth Regulators with Bioprotective Effect
Section: The World of Innovations
Language: Ukrainian
Abstract: With the help of the Dot-blot hybridization the difference in steps of homology between mRNA of control plants and small regulatory si/mi RNA isolated from second-generation plantlets of wheat, corn, soybeans, sugar beets, chickpea, etc. cultivated from the seeds of plants infected and processed by new polycomponent plant growth regulators Regoplant® and Stimpo® in the first generation was found. It is proved that this difference is related to a partial reprogramming of the cell genome under the influence of biostimulators on growing plants with infected backgrounds that turns out in induction of low-molecular si/miRNA with antipathogenic and antiparasitic properties, which are the components of the immune system of a living organism.
Key words: plant stability, immunity, growth biostimulators, si/miRNA, Dot-blot hybridization.
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References:
1. Elbashir S.M., Lendeckel W., Tuscht T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 2001. Vol. 15. P. 188 -200.
https://doi.org/10.1101/gad.862301
2. Hamilton A., Voinnet O., Chappell L. et al. Two classes of short interfering RNA in RNA silencing. TheEMBO Journal. 2002. Vol. 21, No. 17. P. 4671-4679.
https://doi.org/10.1093/emboj/cdf464
3. Lee V., Ahn C, Han J. et al. The nuclear RNase III Drosha initiates microRNA processing. Nature. 2003. Vol. 425. P. 415-419.
https://doi.org/10.1038/nature01957
4. Mourelatos Z., Dostie J., Paushkin S. et al. MiRNPs: a novel class of ribonucleoproteins con taining numerous microRNAs. Genes Dev. 2002. Vol. 16. P. 720-728.
https://doi.org/10.1101/gad.974702
5. Leung R.K.M., Whittaker P.A. RNA interference: from gene silencing to gene-specific therapeutics. Pharmacology and Therapeutics. 2005. No. 107. P. 222-239.
https://doi.org/10.1016/j.pharmthera.2005.03.004
6. Aravin A., Tuscht T. Identification and character ization of small RNAs involved in RNA silencing. FEBS Letters. 2005. V. 579. P. 5830-5840.
https://doi.org/10.1016/j.febslet.2005.08.009
7. Fuller V.L., Lilley C.J., Urwin P.E. Nematode re sistance. New Phytologist. 2008. Vol. 180. P. 27-44.
https://doi.org/10.1111/j.1469-8137.2008.02508.x
8. Bakhetia M., Charlton W.L., Urwin P.E. et al. RNA interference and plant parasitic nematodes. Trends in Plant Science. 2005. Vol. 10, No. 8. P. 362-367.
https://doi.org/10.1016/j.tplants.2005.06.007
9. Gheysen G., Vanholme B. RNAi from plants to nematodes. Trends in Biotechnology. 2006. Vol. 25, No. 3. P. 89-92.
https://doi.org/10.1016/j.tibtech.2007.01.007
10. Knox D.P., Geldhof P., Visser A., Britton C. RNA interference in parasitic nematodes of animals: a reality check? Trends in Parasitology. 2007. Vol. 23, No. 3. P. 105-107.
https://doi.org/10.1016/j.pt.2007.01.007
11. Jian X., Zhang L, Li G. et al. Identification of novel stressregulated microRNAs from Oryza sativa L. Genomics. 2010. Vol. 95. P. 47-55.
https://doi.org/10.1016/j.ygeno.2009.08.017
12. Chen Ft., Hu Z., Zhang H. Identification of MicroR NAs in Wild Soybean (Glycine soja). J. of Inte grative Plant Biology. 2009. Vol. 51, No. 12. P. 1071-1079.
13. Park W., Song R., Messing J., Chen X. CARPEL FACTORY a Dicer Homolog, and HEN1, a Novel Pro tein, Act in microRNA Metabolism in Arabidopsis thaliana in duced silencing complex (RISC), which targets homo logous RNAs for deg radation. Current Biology. 2002. Vol. 12. P. 1484-1495.
https://doi.org/10.1016/S0960-9822(02)01017-5
14. Llave C., Kasschau K.D., Rector M.A., Carring-ton J.C. Endogenous and Silencing-Associat-ed Small RNAs in Plants. Plant Cell. 2002. Vol. 14. P. 1605-1619.
https://doi.org/10.1105/tpc.003210
15. Lu C., Meyers B.C., Green P.G. Construction of small RNA cDNA libraries for deep sequencing. Methods. 2007. Vol. 43. P. 110-117.
https://doi.org/10.1016/j.ymeth.2007.05.002
16. Yang T., Xue L., An L. Functional diversity of miR-NA in plants. Plant Science. 2007. Vol. 172. P. 423-432.
https://doi.org/10.1016/j.plantsci.2006.10.009
17. Ponomarenko S.P., Terek O.M., Gricaenko Z.M. i dr. Bioreguljacija rosta i razvitija ras tenij . «Bioreguljacija mikrobno-rastitel'nyh sistem». Pod. red. Iutinskoj G.A. i Ponomarenko S. P. Gl. 4. Kyiv: Nichlava, 2010. 464 s. [in Russian].
18. Tsygankova V.A., Galkin A.P., Galklna L.O. et al. Gene expression under regulators' stimulation of plant growth and development . «New plant growth regulators: basic research and technologies of application. Ed. S.P. Ponomarenko, H.O. Iutynska. Ch. 3. Kyiv: Nichlava, 2011. 211 p.
19. Cygankova B.A., Andrusevych Ja.V., Bljum Ja.B. Vydilennja z klityn roslyn malyh reguljatornyh si/ miRNA z antynematodnoju aktyvnistju. DAN Ukrai'ny. 2011. No. 9. S. 159-164 [in Ukrainian].
20. Elbashir S.M., Lendeckel W., Tuscht T. RNA interference is mediated by 21 - and 22-nucleotide RNAs. Genes and Development. 2001. Vol. 15. P. 188-200.
https://doi.org/10.1101/gad.862301
21. Hamilton A., Voinnet O., Chappell L., Baulcombe D. Two classes of short interfering RNA in RNA silencing. EMBO Journal. 2002. Vol. 21, No. 17. P. 4671-4679.
https://doi.org/10.1093/emboj/cdf464
22. Lee Y., Ahn C., Han J. et al. The nuclear RNase III Drosha initiates microRNA processing. Nature. 2003. Vol. 425. P. 415-419.
https://doi.org/10.1038/nature01957
23. Mourelatos Z., Dostie J., Paushkin S. et al. MiRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev. 2002. Vol. 16. P. 720-728.
https://doi.org/10.1101/gad.974702
24. Leung R.K.M., Whittaker P.A. RNA interference: from gene silencing to gene-specific therapeutics. Phar macol. Therapeutics. 2005. No. 107. P. 222-239.
https://doi.org/10.1016/j.pharmthera.2005.03.004
25. Aravin A., Tuschl T. Identification and characterization of small RNAs involved in RNA silencing. FEBS Letters. 2005. Vol. 579. P. 5830-5840.
https://doi.org/10.1016/j.febslet.2005.08.009