8(3)07

Nauka innov. 2012, 8(3):50–56
https://doi.org/10.15407/scin8.03.050

Ya.V. Pirko1, A.V. Karelov1,2, N.A. Kozub1,2, I.A. Sozinov2, N.N. Pirko1, A.I. Yemets1, V.I. Korkhovy1, V.T. Koliuchiy3, Ya.B. Blume1, A.A. Sozinov1
1Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv
2Institute of Plant Protection NAAS of Ukraine, Kyiv
3V.M. Remeslo Myronivka Institute of Wheat, UAAS of Ukraine, Kiev region.

 

Implementation of the Methods for Detection of Leaf Rust Resistance Genes in wheat Based on Carrying out Polymerase Chain Reaction

Section: Scientific Basis of Innovation Activity
Language: Ukrainian
Abstract: The molecular genetic methods for identification of leaf rust (Puccinia triticina) resistance gene – Lr34, which is res ponsible for the generic non-specific resistance of wheat to the pathogen were developed. Polymerase chain reaction with codominant marker cssfr5 was carried out to determine the allelic status of the loci Lr34 in the 30 wheat varieties selected in Mironіvka Institute of Wheat of the National Academy of Agrarian Sciences of Ukraine and in the Institute of Plant Physiology and Genetics of National Academy of Sciences of Ukraine. The varieties containing allele Lr34(+), which confers resistance to leaf rust, represented 16,7 % from total number of all tested varieties. Obtained results were handed over to the Mironovka Institute of Wheat for use in breeding process.
Key words: wheat, leaf rust (Puccinia triticina), leaf rust resistance, loci Lr34, polymerase chain reaction (PCR).

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