The technique for estimation of plant condition in unfavorable environment using Western-blot-analysis of the heat shock protein 70 kDa (Hsp70) has been developed. It was shown that Hsp70 level in plant organs (particularly, leaves) can be an integral indicator of plant state and tolerance range under the influence of unfavorable ecological factors. Practical recommendations on Hsp70 use in monitoring of plant natural associations as well as introduced plants and agricultural crops, has been prepared.

1. Kozeko L. Hsp70 level in Sium latifolium leaves in different water environments. Abstr. book PISA conf. 2008 «Responses of plants to environmental stresses» (12-18 May 2008, Elena, Bulgaria). P. 43.
2. Kozeko L., Ovcharenko Yu., Kordyum E. Alcohol dehydrogenase expression in aerial-aquatic plants in response to different water environment. Adv. Agricultural Sci. Problem. 2008. 524. P. 167-171.
3. Sanders B.M. Stress proteins in aquatic organisms: an environmental perspective. Critical Reviews in Toxicology. 1993. 23(1): 49-75.
https://doi.org/10.3109/10408449309104074
4. Werner I., Hinton D.E. Field validation of hsp70 stress proteins as biomarkers in Asian clam (Potamocorbula amurensis): is down regulation an indicator of stress?. Biomarkers. 1999. 4(6): 473-484.
https://doi.org/10.1080/135475099230633
5. Mukhopadhyay I., Nazir A., Saxena D.K., Chowdhuri K.D. Heat shock response: hsp70 in environmental monitoring. J. Biochem. Mol. Toxicology. 2003. 17(5): 249-254.
https://doi.org/10.1002/jbt.10086
6. Timofeev M.A., Shatilina Zh.M., Bedulina D.S. i dr. Oso bennosti primenenija belkov teplovogo shoka v kachestve stress-markerov u vodnyh organizmov na primere bajkal'skih jendemichnyh amfipod. Prikl. biohim. mikrobiol. 2008. 44(3): 343-346 [in Russian].
7. Ireland H.E., Harding S.J., Bonwick G.A., et al. Evaluation of heat shock protein 70 as a biomarker of environmental stress in Fucus serratus and Lemna minor. Biomarkers. 2004. 9(2): 139-155.
https://doi.org/10.1080/13547500410001732610
8. Vierling E. The roles of heat shock proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1991. 42. P. 579-620.
https://doi.org/10.1146/annurev.pp.42.060191.003051
9. Margulis B.A., Guzhova I.V. Belki stressa v jeukarioticheskoj kletke. Citologija. 2000. 42(4): 323-342 [in Russian].
10. Pelham H.R.B. Speculations on the function of major heat shock and glucose-regulated proteins. 1986. 46. P. 959-961.
11. Guy C.L., Li Q.B. The organization and evolution of the spinach stress 70 molecular chaperone gene family. Plant Cell. 1998. 10(4): 539-556.
https://doi.org/10.1105/tpc.10.4.539
12. Nam M.H., Heo E.J., Kim J.Y., et al. Proteome analysis of the responses of Panax ginseng C.A. Meyer leaves to high light: use of electrospray ionization quadrupole-time of flight mass spectrometry and expressed sequence tag data. Proteomics. 2003. 3(12): 2351-2367.
https://doi.org/10.1002/pmic.200300509
13. Zou J., Liu A., Chen X., et al. Expression analysis of nine rice heat shock protein genes under abiotic stresses and ABA treatment. J. Plant Physiol. 2009. 166(8): 851-861.
https://doi.org/10.1016/j.jplph.2008.11.007
14. Jurina N.P. Ustojchivost' k okislitel'nomu stressu u zelenyh vodoroslej: korreljacija s soderzhaniem HSP70B. shaperona hloroplastov. Rastenie i stress: Tez. dokl. Vserossijskogo simp. (Moskva, Rossija, 9-12 nojabrja 2010). Moskva, 2010 [in Russian].
15. Kozeko L.E. Kolichestvennye izmenenija belkov teplovogo shoka Hsp70 i Hsp90 v reakcii prorostkov goroha na kratkovremennoe dejstvie gipergravitacii. Dopovidi NAN Ukraini. 2009. No 1: 140-143 [in Russian].
16. Banti V., Loreti E., Novi G., et al. Heat acclimation and cross-tolerance against anoxia in Arabidopsis. Plant, Cell and Environment. 2008. 31. P. 1029-1037.
https://doi.org/10.1111/j.1365-3040.2008.01816.x
17. Brennecke T., Gellner K., Bosch T.C.G. The lack of a stress response in Hydra oligactis is due to reduced hsp70 mRNA stability. Eur. J. Biochem. 1998. 255(3). P. 703-709.
https://doi.org/10.1046/j.1432-1327.1998.2550703.x
18. Hofmann G.E., Buckley B.A., Airaksinen S., et al. Heatshock protein expression is absent in the Antarctic fish Trematomus bernacchii (Family Nototheniidae). J. Exp. Biol. 2000. 203(15): 2331-2339.