Construction of the Recombinant Yeast Strain of Pichia stipitis with Improved Alcoholic Fermentation of Xylose

TitleConstruction of the Recombinant Yeast Strain of Pichia stipitis with Improved Alcoholic Fermentation of Xylose
Publication TypeJournal Article
Year of Publication2010
AuthorsDmytruk, KV, Kurylenko, OO, Ishchuk, OP, Ubiyvovk, VM, Sibirny, VA, Fedorovych, DV, Sibirny, AA
Short TitleNauka innov.
SectionScientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
The modified form of the xylose reductase (XR) of Pichia stipitis with decreased affinity toward NADPН(H+) was created via site-specific mutagenesis. Recombinant strain of P. stipitis overexpressing the mutated XR characterized by 1,3-fold increase in productivity of xylose alcoholic fermentation was constructed. The rates of alcoholic fermentation of hydrolyzates of wheat bran and sawdust were also improved.
Keywordsalcoholic fermentation, ethanol, Pichia stipitis, xylose, yeast
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