Nauka innov. 2006, 2(6):65-76

Y. V. Sheludko1, Y. R. Sindarovska1, І. М. Gerasymenko1, М. А. Bannikova1, Z. М. Olevynska2, М. Y. Spivak2, N. V. Kuchuk1
1 Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Kyiv
2 D. Zabolotnyi Institute of Microbiology and Virology, NAS of Ukraine, Kyiv


Agrobacterium-Mediated Transient Expression: a Perspective Approach for High-Scale Production of Recombinant Proteins in Plants

Section: Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
Abstract: Agrobacterium-mediated transient expression of foreign genes in plants allows fast obtaining of preparative amounts of recombinant proteins. The manuscript describes optimization of transient expression protocol in planta using a green fluorescent protein (GFP) as a reporter protein, selection of the optimal host species and purification of the preparative amount of the reporter protein. Using the developed protocol we have expressed in N. benthamiana and N. excelsior vector systems harboring interferon or human somototropin genes. The identity of recombinant interferon and somatotropin with the standards has been proved with electrophoretic and immunoblotting analyses. The corresponding biological activities have been found in the crude protein extracts of plants infiltrated with constructions containing interferon or somatotropin gene.
Key words: biotechnology, transient expression, recombinant proteins, Nicotiana benthamiana, Nicotiana excelsior, Agrobacterium.

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