Implementation of Molecular Systems for Identification of Genetic Polymorphism in Winter Wheat to Obtain High-Performance Specialized Varieties

TitleImplementation of Molecular Systems for Identification of Genetic Polymorphism in Winter Wheat to Obtain High-Performance Specialized Varieties
Publication TypeJournal Article
Year of Publication2016
AuthorsMorgun, BV, Stepanenko, AI, Stepanenko, OV, Bannikova, MO, Holubenko, AV, Nitovska, IO, Maystrov, PD, Grodzinsky, DM
Short TitleNauka innov.
SectionScientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
The molecular genetic polymorphism detection systems to screen the presence of alleles in winter wheat 100 varieties were developed. Polymerase chain reactions were deployed to identify relevant genes. The level of allele prevalence of low and medium activity of polyphenol oxidase enzymes was defined and the validation was carried out. Wheat varieties carrying rye 1AL.1RS, 1BL.1RS translocations were characterized and those containing recessive allele of Tamyb10 gene, with Stb4 gene resistance to Septoria linked to polymorphic locus Xgwm111. Waxy wheat variety was discovered and other varieties carrying atypical functional Wx-B1e allele. Characteristics of 100 elite and perspective varieties of wheat were compiled for the presence of alleles of genes determining grain quality (genes PPO, Tamyb10-A1, Wx), resistance to biotic and abiotic stress (rye translocative material, Tamyb10-A1, Stb4).
Keywordsallele, grain quality characteristics, polymerase chain reactions, primer, wheat

1. Lukaszewski A.J. Frequency of 1RS.1AL and 1RS.1BL translocations in United States wheat / A.J. Lukaszewski. Crop Sci. 1990. V.30: 1151-1153.
2. McIntosh R.A. Cataloge of gene symbols for wheat. R.A. McIntosh, G. Hart, M. Gale. Proc. of the 8th Intern. Wheat Genet. Symp. Eds Z.S. Li, Z.Y. Xin. Beijing, China. 1993: 1333-1500.
3. Rabinovich S.V. Importance of wheat-rye translocations for breeding modern cultivars of Triticum aestivum L. S.V. Rabinovich. Euphytica. 1998. V.100: 323-340.
4. Schlegel R. Current list of wheats with rye and alien introgression. Shlegel. 2010. V.5-8: 1-14.
5. Singh N.K. Linkage mapping of genes for resistance to leaf,steam and stripe rust and secalins on the short arm of rye chromosome 1R. N.K. Singh, K.W. Shepherd, R.A. McIntosh. Theor. Appl. Genet. 1990. V.80: 609-616.
6. Catalogue of gene symbols for wheat. Proc. of the 10th Intern. Wheat Genet. Symp. R.A. McIntosh, Y. Yamazaki, K.M. Devos [et al.] Eds N.E.Pogna, M. Romano, G. Galterio. Paestum, Italy, 2003: 1-6.
7. Meltz G., Schlegel R., Thiele V. Genetic linkage map of rye. Theor. Appl. Genet. 1992. V.83: 33-45.
8. Kozub N.O., Sozinov I.O., Koljuchyj V.T. ta in. Identyfikacija 1AL/1RS translokacii' u sortiv m'jakoi' pshenyci ukrai'ns'koi' selekcii'. Cytologyja y genetyka. 2005. 39(4): 20-24 [in Ukrainian].
9. Hoffmann B. Alteration of drought tolerance of winter wheat caused by translocation of rye chromosome segment 1R. Cereal Res. Commun. 2008. V.36: 269-278.
10. Kim Y.-Y., Kim D.-Y., Donghwan S. et al. Expression of the novel wheat gene TM20 confers enhanced cadmium tolerance to bakers' yeast. J. of Biologocal Chemistry. 2008. 283(23): 15893-15902.
11. Kozub N.A., Sozynov Y.A., Sozynov A.A. Soprjazhennost' 1BL/1RS translokacyy s kachestvennіmy y kolychest vennыmy pryznakamy u mjagkoj pshenycі T. aestivum. Cytologyja y genetyka. 2001. 35(5): 74-80 [in Uk rainian].
12. Zhou Y., He Z.H., Sui X.X. et al.Genetic improvement of grain yield and associated traits in the Northern China winter wheat region from 1960 to 2000. Crop Sci. 2007. V.47: 245-253.
13. Rybalka O.I. Jakist' pshenyci ta i'i' polipshennja. Kyiv: Logos, 2011 [in Ukrainian].
14. Syvolap Ju.M., Chebotar S.V., Sudarchuk L.V. Detekcija 1RS.1AL, 1RS.1BL ta modyfikovanoi' translokacij za 1RS hromosomoju u selekcijnyh form m'jakoi' pshenyci. Metodychni rekomendacii'. Odesa, 2011 [in Ukrainian].
15. Gupta R.B., Shepherd K.W. Identification of rye chromo some 1R translocations and substitutions in hexaploid wheats using storage proteins as genetic markers. Plant Breeding. 1992. V.109: 130-140.
16. Berzonsky W.F., Francki G. Biochemical, molecular and cytogenetic technologies for characterizing 1RS in wheat: a review. Euphytica. 1999. 108: 1-15.
17. Dexter J.E., Preston K.R., Matsuo R.R. et al. De ve lopment of a high extraction flour for the GRL Pilot Mill to evaluate Canadian wheat potential for the Chinese market. Can Inst Food Sci Technol. 1984. 14: 253-259.
18. Feillet P., Autran J.C., Icard-Verniere C. Pasta brownness: an assessment. J Cereal Sci. 2000. V. 32: 215-233.
19. Simeone R., Pasqualone A., Clodoveo M.L. et al. Genetic mapping of polyphenol oxidase in tetraploid wheat. Cell Mol Biol Lett. 2002. V.7: 763-769.
20. McCallum J.A., Walker J.R.L. O-diphenol oxidase ac ti vi ty, phenolic content and colour of New Zealand whe ats, flours and milling streams. J Cereal Sci. 1990. V.12: 83-96.
21. McCallum J.A., Walker J.R.L. Proanthocyanidins in wheat bran. Cereal Chem. 1990. 67(3): 282-285.
22. Fuerst E.P., Xu S.S., Beecher B. Genetic characterization of kernel polyphenol oxidase in wheat and related species. J Cereal Sci. 2008. V.48: 359-368.
23. Jukanti A.K., Bruckner P.L., Fischer A.M. Evaluation of wheat polyphenol oxidase genes. Cereal Chem. 2004. V.81: 481-485.
24. Massa A.N., Beecher B., Morris C.F. Polyphenol oxidase (PPO) in wheat and wild relatives: molecular evidence for a multigene family. Theor Appl Genet. 2007. V.114: 1239-1247.
25. Demeke T., Morris C.F., Campbelle K.J. et al. Wheat Polyphenol Oxidase. Crop Sci. 2001. 41(6): 1750-1757.
26. Raman R., Raman H., Martin P. Functional gene markers for polyphenol oxidase locus in bread wheat (Triticum aestivum L.). Molecular Breeding. 2007. 19(4): 315-328.
27. Sun Y., He Z., Ma W. et al. Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.). Functional & Integrative Genomics. 2011. 11(1): 85-93.
28. Vatanabe N., Takeuchi A., Nakayama A. Vatanabe N. Inheritance and chromosomal location of the homoeologous genes affecting phenol colour reaction of kernels in durum wheat. Euphytica. 2004. V.139: 87-93.
29. Beecher B.S., Skinner D.Z. Molecular cloning and expression analysis of multiple polyphenol oxidase genes in developing wheat (Triticum aestivum) kernels. J Cereal Sci. 2011. V.53: 371-378.
30. Sun D.J., He Z.H., Xia X.C. et al. A novel STS marker for polyphenol oxidase activity in bread wheat. Mol Breed. 2005. V.16: 209-218.
31. He X.Y., He Z.H., Zhang L.P. et al. Allelic variation of polyphenol oxidase (PPO) genes located on chro mo somes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theor Appl Genet. 2007. V.115: 47-58.
32. Beecher B.S., Carter A.H., See D.R. Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.). Theor Appl Genet. 2012. V.124: 1463-1473.
33. Flintham J.E. Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res. 2000. V.10: 43-50.
34. Warner R.L., Kudrna D.A., Spaeth S.C. et al. Dormancy in white-grain mutants of Chinese Spring wheat (Tri ticum aestivum L.). Seed Sci Res. 2000. V.10: 51-60.
35. Himi E., Mares D.J., Yanagisawa A. et al. Effect of grain colour gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. J Exp Bot. 2002. 53(374): 1569-1574.
36. Himi E., Nisar A., Noda K. Colour genes (R and Rc) for grain and coleoptile upregulate flavonoid biosynthesis genes in wheat. Genome. 2005. 48(4): 747-754.
37. Himi E., Noda K. Isolation and location of three ho moeologous dihydroflavonol-4-reductase (DFR) genes of wheat and their tissue-dependent expression. J Exp Bot. 2004. 55(396): 365-375.
38. Shaner G., Finney R.E. Weather and epidemics of Septoria leaf blotch of wheat. Phytopathology. 1976. V.66: 781-785.
39. Adhikari T.B., Cavaletto J.R., Dubcovsky J. et al. Mo lecu lar mapping of the stb4 gene for resistance to Septoria tritici blotch in wheat. Phytopathology. 2004. V.94: 1198-1206.
40. Brading P.A., Verstappen E.C.P., Kema G.H.J. et al. A gene-for-gene relationship between wheat and Mycospha erella graminicola, the Septoria tritici blotch pathogen. Phytopathology. 2002. V.92: 439-445.
41. McCartney C.A. Inheritance and chromosomal location of race-specific resistance to Mycosphaerella graminicola in wheat. Ph.D. thesis. University of Manitoba, Win nipeg, Canada. 2002.
42. Kopus' M.M., Ignat'eva N.G., Vasjushkina N.E. i dr. Gene ticheskij polimorfizm amiloliticheskih fermentov zerna pshenicy i genetika fermentov biosinteza krahmala. Zer novoe hozjajstvo Rossii. 2009, no 4: 23-27 [in Russian].
43. Chao S., Sharp P. Chao S. RELP-based genetic map of wheat homeologous group 7 chromosomes. Teor. Appl. Genet. 1989. V.78: 495-504.
44. Rodriguez-Quijano M., Nieto-Taladriz M.T., Carrillo J.M. Polymorphism of waxy proteins in Iberian he xa ploidwheats. Plant Breeding. 1998. V.117: 341-344.
45. Saito M., Vrinten P., Nakamura T. DNA markers for identifying waxy mutations and improving noodle quality in wheat. JARQ. 2010. 44(2): 109-115.
46. Zhao X.C., Sharp P.J., Crosbie G. et al. A single genetic locus associated with starch granule properties in a cross between wheat cultivars of disparate noodle quality. J. Ce real Sci. 1998. V.27: 7-13.
47. Stewart C.N., Via L.E. Stewart C.N. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Bio Techniques. 1993. 14(5): 748-749.
48. Rybalka O.I., Chervonis M.V., Shherbyna Z.V. Ge ne tychnyj polimorfizm klejkovynnyh bilkiv zerna, pov'ja za nyh z jakistju boroshna pshenyci: metody identyfikacii'. Zb. nauk. prac' SGI NCNS. 2007. 10(50): 52-71 [in Ukrainian].
49. Stepanenko A.I. Avtoreferat, 2015 [in Ukrainian].
50. Vlasenko V.A. Stvorennja vyhidnogo materialu dlja adaptyvnoi' selekcii' i vyvedennja vysokoproduktyvnyh sortiv pshenyci v umovah Lisostepu Ukrai'ny. Avtoref. dys. dokt. s.-g. nauk. Odessa, 2008 [in Ukrainian].
51. Rogowsky P.M., Shepherd K.W., Langridge P. Poly mera se chain reaction based mapping of rye involving repeated DNA sequences. Genome. 1992. V.35: 621-626.
52. Saal D., Wricke G. Development of simple sequence repeat markers in rye (Secale cereale L.). Genome. 1999. V.42: 964-972.
53. Vanzetti L.S., Pflüger L.A., Rodrίguez-Quijano M. et al. Genetic variability for waxy genes in Argentinean bread wheat germplasm. Electronic J. Biotechnol. 2009. V.12: 1-9.
54. Saito M., Vrinten P., Ishikawa G. et al. A novel codo mi nant marker for selection of the null Wx-B1 allele in wheat breeding programs. Mol. Breeding. 2009. V.23: 209-217.
55. Divashuk M.G., Klimushina M.V., Karlov G.I. Mole ku ljarno-geneticheskaja harakteristika allelja Wx-B1e mjag koj pshenicy i primenimost' DNK markerov dlja ego iden ti fika cii. Genetika. 2011. 47(12): 1611-1615 [in Russian].
56. Vrinten P., Nakamura T., Yamamori M. Molecular charac terization of waxy mutations in wheat. Mol. General Genet. 1999. V.261: 463-471.