Nauka innov. 2005, 1(3):70-75

S. S. Maliuta, G. D. Telegeev, M. V. Dybkov, D. O. Miroshnychenko, G. V. Yelska
Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv


Development of Test-System for Ph-Leukaemia Diagnostics by Polymerase Chain Reaction

Section: Scientific and Technical Innovative Projects of National Academy of Sciences of Ukraine
Language: Ukrainian
Abstract: The hallmark of tumor growth for the majority of patient with chronic myelogenous leukemia is Philadelphia chromosome (Ph') – a product of reciprocal translocation of 9 and 22 chromosomes. At the molecular level it is connected with bcr/abl-fusion gene. This paper presents data of development of national test-system for detection of hybrid gene with PCR method. It was shown that specific combination of primers used allow to advance a probability to obtain the correct data.
Key words: Ph-positive leukaemia, BCR/ABL, diagnostics, PCR.

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